QUANTIFICATION OF VARIOUS ISOFLAVONES EXTRACTED FROM RED CLOVER
Keywords:
plant material, phytoestrogens, isoflavonoids, extraction methods, extract of red clover flowers.Abstract
This article describes methods of new ecological extraction of isoflavonoids from
red clover flowers based on the use of deep eutectic solvents (NADES), consisting of citric acid
and choline chloride are described, with simultaneous extraction of associated biologically active
compounds. Data are given on. An analysis of the total polyphenols and antioxidants, including
isoflavones, showed that NADES with a 30% water concentration was the most effective for
extraction.
References
Wei Z, Zu Y, Fu Y, Wang W, Luo M, Zhao C, Pan Y. Ionic liquids-based microwave-assisted
extraction of active components from pigeon pea leaves for quantitative analysis. Separation and
Purification Technology. 2013 Jan 4;102:75-81.
Huang J, Guo X, Xu T, Fan L, Zhou X, Wu S. Ionic deep eutectic solvents for the extraction
and separation of natural products. Journal of Chromatography A. 2019 Mar 30.
Delmonte P, Perry J, Rader JI. Determination of isoflavones in dietary supplements
containing soy, Red Clover and kudzu: extraction followed by basic or acid hydrolysis. Journal of
Chromatography A. 2006 Feb 24;1107(1-2):59-69.