CULTIVATION OF COLLECTION STRAINS OF ORTHOPOXVIRUSES IN CELL CULTURES
Keywords:
cowpox virus, vaccinia virus, strains, cell culture, biological activity, passage.Abstract
The study of orthopoxviruses was steadfastly pursued until the complete disappearance of the variola virus in the 1980s. However, after the 1980s, research on orthopoxviruses
slowed down due to their beautiful relevance. In recent years, in connection with the detection
of cowpox and monkeypox viruses, not only in animal populations, but also among humans, the
study of orthopoxviruses has become more relevant.
It is known from earlier studies that some smallpox vaccines have been successfully used to
eradicate smallpox, although in most cases the safety of the vaccines has so far been of concern to
many virologists. The main reason was that the vaccine was made from animal tissues and many
virologists decided to use cell cultures when cultivating viruses. However, to date, the solution to
this problem is still open. In this regard, this article presents the results of determining some of the
cultural properties of collection strains of orthopoxviruses.
As a result of the studies, it was found that vaccinia viruses (VAV) and cowpox (VOK) reproduce freely in the cells of the kidney of the lamb (LA) and in the cells of the kidney of the African
green monkey (Vero). At the same time, the cytopathic effect (CPE) of the virus on the cell monolayer occurred quickly, and the period of virus cultivation was reduced from 120 h to 24 h, while
the biological activity remained unchanged and amounted to 5.100.10 lgTCID50/cm3
for the WOK
strain, for the WOV strain 5.15± 0.10 lgTCID50/cm3
for the WOK strain, for the WOV strain 5.15±
0.11 lgTCID50/cm3.
Sequential passaging of viruses in these culture systems did not lead to a significant change
in the biological activity of the obtained virus-containing material. The results of these studies are
primary incomplete data on the cultural properties of these strains of the virus.
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