SELECTION OF PRIMERS FOR THE RPOB GENE TO THE PATHOGEN MYCOPLASMA MYCOIDES SUBSP. CAPRI
Keywords:
goat contagious pleuropneumonia, gene, pathogen.Abstract
The article describes the results of studies on the selection of specific primers
based on the rpoB gene of the causative agent of contagious goat pleuropneumonia (CBPP). For
the selection of specific primers, NCBI resources were used: BLAST nucleotide and PRIMER
BLAST. Primer synthesis was carried out by chemical preconcentration on a Synthesyzer H-16
device (K&A Laborgeraete). As a result of the work carried out, three pairs of primers for the rpoB
gene were selected, satisfying in all respects.
References
Antunes N.T. In Vitro Susceptibilities of Mycoplasma putrefaciens Field Isolates/ Antunes N.T. // Antimicrobial Agents and Chemotherapy, 2007- Vol. 51.- No. 9.- PP. 3452-3454.
Carson J.L. Cell structural and functional elements. In: Maniloff J., McElhaney R.N., Finch L.R., Basema J.B. (Editors) Mycoplasmas: Molecular Biology and Pathogenesis, Washington DC: American Society for Microbiology, USA, 1992.- PP. 63-77.
Mohd. Iqbal Yatoo, Oveas Raffiq Parray, Shah Tauseef Bashir, Muheet, Riyaz Ahmed Bhat, Arumugam Gopalakrishnan, Kumaragurubaran Karthik, Kuldeep Dhama & Shoor Vir Singh 2019, Contagious caprine pleuropneumonia–a comprehensive review // Veterinary Quarterly, Vol. 39.- NO. 1.- 1–25.
Jones G. E. et al. A review of experiments on the reproduction of chronic pneumonia of
sheep by the use of pneumonic lung homogenate suspensions. //Vet Bull, 1986.- No. 56.- PP. 251- 253.
Avron A. Mycoplasma stimulates the production of oxidative radicals by murine peritoneal
macrophages. J. Leukocyte Biol, 1995.- No. 57.- PP. 264-268.
Awan M. A. Isolation and identification of Mycoplasmas from pneumonic lungs of goats. J. App. Em, 2004.- PP. 45-50.
Bonnet F. DNA relatedness between field isolates of Mycoplasma F38 group, the agent of contagious caprine pleuropneumonia, and strains of Mycoplasma capricolum. Int. Journal of Systematic Bacteriology, 1993.- No. 43.- PP. 597-602.
Houshaymi B.M. Studies on strains of M. capricolum subsp. capripneumoniae isolated from
outbreaks of contagious caprine pleuropneumonia. Small Ruminant Res, 2000.- vol.45.- No. 2.- PP. 139-143.
Tariq M.A. Studies on the incidence, epizootology and development of effective vaccines for the control of contagious caprine pleuropneumonia, contagious agalactia in sheep and goats in Balochistan (Annual report), Livestock Department Government of Balochistan, Pakistan, 1980.- PP. 132-139.
F.Thiaucourt and G.Bölske, Contagious caprinepleuropneumonia and other pulmonary mycoplasmoses of sheep and goats Rev.sci.tech.Off.int.Epiz.,1996.-15(4).-1397-1414.
F. Thiaucourt, L. Manso-Silvan, W. Salah, V. Barbe, B. Vacherie, D. Jacob and others/ Mycoplasma mycoides, from “mycoides Small Colony” to “capri”. A microevolutionary perspective/ BMC Genomics. 2011; 12: 114.
